Third-Generation Nanopore Sequencing for Post-Transplant Chimerism Monitoring.

Real-time Nanopore sequencing replacing STR/qPCR/NGS for HSCT chimerism monitoring — reduced turnaround, applicable in urgent clinical contexts

Post-transplant chimerism monitoring is a key clinical tool for early detection of graft rejection and relapse. Current strategies (STR, qPCR, dPCR, NGS) are limited by turnaround time and batch dependency. Third-generation Nanopore sequencing, with real-time sequencing, offers a faster alternative with analytical robustness comparable to NGS, applicable in urgent clinical contexts such as post-transplant aplasia or relapse.

Synthesis written by Geno'X. For the full original abstract, please refer to the source publication.

Applying Nanopore to chimerism monitoring is a logical — and welcome — progression for transplant centers that need results within 24 hours in critical clinical situations. It is a concrete example of the added value of clinical long-read sequencing beyond rare disease diagnosis.

Clinical impact: 3/3 · Evidence strength: 2/3 · Novelty: 1/2 · Sample size: 1/1 · Publication status: 0/1 → Total: 7/10