pAnno: a comprehensive, precise, and fast proteogenomic workflow for the discovery of novel coding regions.

Integration of genomic, transcriptomic and proteomic data for discovery of novel coding regions

PAnno is an end-to-end proteogenomic workflow integrating genomic, transcriptomic, and proteomic data to identify unannotated coding regions, protein isoforms, and HLA peptides. The pipeline generates customized sequence databases and enriches them through mass spectrometry analysis. Applied to HLA peptide detection in lung cancer, pAnno achieves a 34-fold improvement over standard tools. A web server is available for deployment.

Synthesis written by Geno'X. For the full original abstract, please refer to the source publication.

Proteogenomics remains niche in routine diagnostics, but pAnno offers a concrete pathway to enrich variant annotation and improve neoantigen identification in precision oncology. The claimed speed and availability of a web server are genuine assets for clinical laboratory adoption.

Clinical impact: 3/3 · Evidence strength: 3/3 · Novelty: 2/2 · Sample size: 1/1 · Publication status: 1/1 → Total: 10/10