Accuracy of nanopore sequencing technology for rapid diagnosis of tuberculous mediastinal and hilar lymphadenopathy.

Direct M. tuberculosis identification by ONT sequencing from EBUS-TBNA samples

This study evaluates the accuracy of Oxford Nanopore Technology (ONT) sequencing for rapid diagnosis of tuberculous mediastinal and hilar lymphadenopathy from EBUS-TBNA samples — clinically difficult specimens. ONT enables direct real-time identification of M. tuberculosis from raw clinical samples, bypassing the delays of conventional cultures and the sensitivity limitations of PCR on this specimen type. Results demonstrate high diagnostic accuracy, positioning ONT as a practical solution for front-line respiratory infectious disease, including in resource-limited settings.

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This application of Nanopore long-read sequencing to clinically difficult specimens (mediastinal nodes via EBUS-TBNA) illustrates the diagnostic versatility of ONT beyond constitutional genomics. The portability and speed of ONT make it a serious candidate for resource-limited settings or emergency diagnostic situations. Another illustration of the clinical maturation of long-read sequencing beyond conventional WGS.

Clinical impact: 2/3 · Evidence strength: 2/3 · Novelty: 1/2 · Sample size: 1/1 · Journal quality: 0/1 → Total: 6/10