Spatially resolved m6A profiling across tissues using m6A-ARTR-DBiT.

m6A-ARTR-DBiT: spatially resolved transcriptome-wide m6A profiling preserving native tissue context

This Nature Methods study introduces m6A-ARTR-DBiT, a spatial profiling method for the epitranscriptomic m6A (N6-methyladenosine) modification at transcriptome-wide scale while preserving native tissue context. m6A plays diverse regulatory roles on RNA, but its spatial distribution within tissues remained largely unexplored. The method combines reverse-transcription-based detection with deterministic barcoding in tissue.

Synthesis written by Geno'X. For the full original abstract, please refer to the source publication.

Spatial transcriptomics is booming, but spatial epitranscriptomics was still in its infancy. m6A-ARTR-DBiT opens a new resolution level — spatial distribution of RNA modifications in tissue — with potential applications in disease pathophysiology and tissue-specific diagnostics.

Clinical impact: 1/3 · Evidence strength: 2/3 · Novelty: 2/2 · Sample size: 1/1 · Publication status: 1/1 → Total: 7/10